Development And Validation Of Two Stability- Indicating Uv-Spectrophotometric Methods For The Determination Of Repaglinide In Bulk And Dosage Forms

Two stability indicating UV-spectrophotometric methods were developed and validated for the determination of repaglinide with its degradation products. Assay of repaglinide in 0.1M NaOH and 0.1M HCl were achieved at 216nm and 243nm respectively. The correlation was in the range of 1.0 – 25 μg mL (r = 0.9999) for 0.1M NaOH and 2.0 – 40 μg mL (r = 0.9999) for 0.1M HCl with molar absorptivity values of 2.02 × 10 L mol cm and Sandell sensitivity of 0.0224 μg cm for 0.1M NaOHand 1.17 × 10 L mol cm and Sandell sensitivity of 0.0386 μg cm for 0.1M HCl. The detection limit and quantification limit are calculated to be (0.13, 0.39 μg mL) and (0.18 and 0.54 μg mL) for 0.1M NaOH and 0.1M HCl respectively. The method was validated for accuracy, precision, robustness, selectivity and applied for marketed formulations with less than 2% relative error and standard deviation.